Heat tolerance genetics identified for chickpea

Biodiversity delivers heat tolerance traits for chickpea

Crops
Chickpea seed with heat tolerant characteristics has made its way to Australian plant breeders, who are investigating how the crop can tackle this stress at flowering time. PHOTO Angela Pattison

Chickpea seed with heat tolerant characteristics has made its way to Australian plant breeders, who are investigating how the crop can tackle this stress at flowering time. PHOTO Angela Pattison

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Chickpea resilience boosted with inclusion of heat tolerance genetics into Australian lines.

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Pre-breeders working to improve the heat tolerance of chickpea during flowering have delivered the first seed from this research for use by breeders.

The seed was harvested in 2019 by researchers in the GRDC-invested Australian Research Council (ARC) Industrial Transformation Research Hub called Legumes for Sustainable Agriculture.

Researcher Dr Angela Pattison, from the University of Sydney, says the new heat tolerance genetics were sourced from a wide array of biodiverse material, including:

  • lines collected from hot, dry regions of the chickpea growing world;
  • genetic resources held at the Australian Grains Genebank (AGG), at Horsham in Victoria; and
  • the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) reference set sourced from India and held at the University of Adelaide.

This material was screened for heat tolerance in the paddock during summer, in addition to using:

  • delayed sowing;
  • in-field heat chambers; and
  • glasshouse work.

That work was performed primarily at the Institute of Plant Breeding at Narrabri, in New South Wales, where exotic germplasm was tested for yield under heat stress.

Screening also included measuring flower fertility - given that it's known that a leading cause of yield losses is due to pollen sterility once temperatures exceed 30°C - with female fertility becoming an additional issue at higher temperatures.

We are seeing a lot of diversity in the ability of our germplasm to withstand extreme temperatures. There's plenty of room for breeders to make gains, which is the good news. - University of Sydney researcher Dr Angela Pattison

Using global experience

University of Sydney researcher, Dr Angela Pattison. PHOTO Angela Pattison

University of Sydney researcher, Dr Angela Pattison. PHOTO Angela Pattison

Starting in 2016, Dr Pattison crossed the best performing lines with Australian adapted material to facilitate its use by breeders.

This was necessary, as some of the lines that express enhanced heat tolerance were "a bit wild and woolly".

This pre-breeding work has now resulted in 20 families of lines with promising levels of enhanced heat tolerance. These are at different stages of development, with the number increasing every year.

"We are seeing a lot of diversity in the ability of our germplasm to withstand extreme temperatures," Dr Pattison says.

"There's plenty of room for breeders to make gains, which is the good news."

Adaptation to local conditions

Early flower on a chickpea plant containing heat tolerance genes. PHOTO Angela Pattison

Early flower on a chickpea plant containing heat tolerance genes. PHOTO Angela Pattison

As the seed reaches breeders, they will make additional crosses to bring the germplasm up to Australian standards for cultivation.

This includes adding in high levels of disease resistance and improving height to optimise harvesting with Australian machines.

Getting a robust estimate of the yield benefit associated with the new genetics was initially difficult, given short row trials and a low supply of seed.

However, the 2019 trials were different - with machine-sown plots providing a better idea of yield performance.

While Dr Pattison does not yet have figures associated with different families, she has seen lines that perform just as well as the Australian parent under 'normal' conditions - and better under hot conditions.

Additionally, she is exploiting opportunities to combine different sources of heat tolerance together into the same lines in an attempt to further boost yield potential.

What the future holds

Throughout the project, chickpea breeders - such as Kristy Hobson and Damien Scanlon - had the opportunity to walk through the field trials and examine the plants.

Once the yield data becomes available, final decisions will be made about which lines they want to include in their breeding programs.

"What they look for are plants that are healthy, happy, vigorous, flowering/podding at the correct time, and are machine harvestable," Dr Pattison says.

The Legumes for Sustainable Agriculture is a five-year collaborative research program based at the University of Sydney and headed by Associate Professor Brent Kaiser. It includes:

  • the University of Adelaide;
  • The University of Western Australia;
  • Flinders University;
  • the Australian National University;
  • La Trobe University; and
  • NC State University in the USA.

Prebreeding material is also being tested by the Northern Australian Crop Research Alliance (NACRA) in the Ord River area of Western Australia.

See also:

GRDC Research Code US00083

More information: Angela Pattison, angela.pattison@sydney.edu.au; Legumes for Sustainable Agriculture program, https://www.legumehub.com

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